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Article

Catalytic efficiency and sequence selectivity of a restriction endonuclease modulated by a distal manganese ion binding site.

Journal of molecular biology - 2 Mar 2001

Sam M D, Horton N C, Nissan T A, Perona J J

Abstract excerpt

Crystal structures of EcoRV endonuclease bound in a ternary complex with cognate duplex DNA and manganese ions have previously revealed an Mn(2+)-binding site located between the enzyme and the DNA outside of the dyad-symmetric GATATC recognition sequence. In each of the two enzyme subunits, this metal ion bridges between a distal phosphate group of the DNA and the imidazole ring of His71. The new metal- binding...

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Source

Identifiers

PMID: 11243793
DOI: 10.1006/jmbi.2000.4434

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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