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Article

Active TEM-1 beta-lactamase mutants with random peptides inserted in three contiguous surface loops.

Protein science : a publication of the Protein Society - 1 Oct 2006

Mathonet Pascale, Deherve Julie, Soumillion Patrice, Fastrez Jacques

Abstract excerpt

Engineering of alternative binding sites on the surface of an enzyme while preserving the enzymatic activity would offer new opportunities for controlling the activity by binding of non-natural ligands. Loops and turns are the natural substructures in which binding sites might be engineered with this purpose. We have genetically inserted random peptide sequences into three relatively rigid and contiguous loops of...

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Identifiers

PMID: 16963643
DOI: 10.1110/ps.062303606
PMCID: PMC2242396

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Source receipt: pubmed, retrieved 7 Sept 2026.

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