Article
Diversified sequences of peptide epitope for same-RNA recognition.
Proceedings of the National Academy of Sciences of the United States of America - 1 Nov 1993
Kim S, Ribas de Pouplana L, Schimmel P
Abstract excerpt
We replaced an essential RNA-binding, 30-amino acid helix-loop in an Escherichia coli tRNA synthetase with an inactive and simplified "generic" sequence having 23 of the 30 amino acids as alanine and serine. Wild-type residues were restored in random combinations to generate a library with a sequence complexity of about 1.9 x 10(7). Active molecules were obtained by genetic selection at a frequency of...
Topics
- Anticodon
- Binding Sites
- Calorimetry
- Epitopes
- Escherichia coli
- Genes, Bacterial
- Genetic Variation
- Geobacillus stearothermophilus
- Methionine-tRNA Ligase
- Mutagenesis, Site-Directed
- Point Mutation