Article
Engineering surface loops of proteins--a preferred strategy for obtaining new enzyme function.
Trends in biotechnology - 1 May 1994
el Hawrani A S, Moreton K M, Sessions R B, Clarke A R, Holbrook J J
Abstract excerpt
A prerequisite for the rational redesign of enzymes is that altering amino acids in an attempt to obtain new biological function does not unexpectedly alter the globular, natural framework of the native protein on which the design is being executed. The results of combinatorial-mutagenesis strategies suggest that random variation of amino acid sequence is most easily tolerated at the solvent-exposed surfaces of a...
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