Amplification of a beta-haemoglobin sequence in individual human oocytes and polar bodies.

doi:10.1016/0140-6736(90)91060-n

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Article

Amplification of a beta-haemoglobin sequence in individual human oocytes and polar bodies.

Lancet (London, England) - 28 Apr 1990

Monk M, Holding C

Abstract excerpt

A 680 base-pair sequence of the human beta-haemoglobin gene was reproducibly amplified in individual unfertilised human oocytes and in first polar bodies isolated from them. Specificity and sensitivity of amplification were achieved by two sequential reactions with two sets of primers, amplifying...

Topics

Amino Acid Sequence
Anemia, Sickle Cell
Beta-Globulins
DNA
Homozygote
Humans
Meiosis
Molecular Sequence Data
Mutation
Nucleic Acid Amplification Techniques
Oocytes
Polymorphism, Restriction Fragment Length

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