Article
Amplification of a beta-haemoglobin sequence in individual human oocytes and polar bodies.
Lancet (London, England) - 28 Apr 1990
Monk M, Holding C
Abstract excerpt
A 680 base-pair sequence of the human beta-haemoglobin gene was reproducibly amplified in individual unfertilised human oocytes and in first polar bodies isolated from them. Specificity and sensitivity of amplification were achieved by two sequential reactions with two sets of primers, amplifying first a 725 base-pair sequence and secondly a 680 base-pair sequence from within the first amplified fragment. A...
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