Article
Polar body diagnosis for hemophilia a using multiplex PCR for linked polymorphic markers.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society - 1 Mar 2005
Tomi Diana, Griesinger Georg, Schultze-Mosgau Askan, Eckhold Juliane, Schöpper Beate, Al-Hasani Safaa, Diedrich Klaus, Schwinger Eberhard
Abstract excerpt
Preimplantation genetic diagnosis (PGD) is usually performed on blastomeres. In Germany, the only possibility to perform PGD is by analysis of polar bodies. We performed PGD using polar bodies in a woman who is a carrier of hemophilia A. Multiplex PCR followed by nested fluorescent PCR for five linked polymorphic markers was established. From 11 analyzed polar bodies, only 1 showed alleles linked to the mutation....
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