Article
Co-amplification of the cystic fibrosis delta F508 mutation with the HLA DQA1 sequence in single cell PCR: implications for improved assessment of polar bodies and blastomeres in preimplantation diagnosis.
Prenatal diagnosis - 1 Dec 1993
Wu R, Cuppens H, Buyse I, Decorte R, Marynen P, Gordts S, Cassiman J J
Abstract excerpt
We have developed a heminested PCR (polymerase chain reaction) method, performed on single cells, for the analysis of the most common cystic fibrosis (CF) mutation (delta F508). As a quality control, the polymorphic exon 2 of the HLA DQA1 locus was co-amplified from the same cell. With a non-radioactive reverse dot-blot assay, the genotype of these two loci could be determined. Experiments on 98 single...
Topics
- Base Sequence
- Blastomeres
- Cystic Fibrosis
- Cystic Fibrosis Transmembrane Conductance Regulator
- Embryonic Development
- Exons
- Female
- Fibroblasts
- HLA-DQ Antigens
- HLA-DQ alpha-Chains