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Article

Direct electrophoretic detection of the allelic state of single DNA molecules in human sperm by using the polymerase chain reaction.

Proceedings of the National Academy of Sciences of the United States of America - 1 Jun 1990

Li H, Cui X, Arnheim N

Abstract excerpt

We have developed a procedure that allows the detection of polymerase chain reaction (PCR) products derived from a single target DNA molecule in a human sperm without using radioactive probes. With this method, three genetic loci present in a single sperm can be amplified simultaneously. The amplification procedure is specific as well as efficient and permits detection of the PCR product by ethidium bromide...

Topics

Alleles
Base Sequence
DNA
Electrophoresis, Polyacrylamide Gel
Genetic Variation
Humans
Male
Molecular Sequence Data
Nucleic Acid Amplification Techniques
Oligonucleotide Probes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Recombination, Genetic

Restriction Mapping

Spermatozoa

Source

Open in PubMed Open full text

Identifiers

PMID: 1972276
DOI: 10.1073/pnas.87.12.4580
PMCID: PMC54160

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1972276-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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