Article
Determination of the number of polypeptide subunits in a functional VDAC channel from Saccharomyces cerevisiae.
Journal of bioenergetics and biomembranes - 1 Feb 1992
Peng S, Blachly-Dyson E, Colombini M, Forte M
Abstract excerpt
Genes encoding VDAC proteins containing specific site-directed amino acid alterations were introduced into wild-type Saccharomyces cerevisiae. The mutant VDAC proteins form channels with ion selectivities very different from that of the wild-type channel. Therefore, the resulting yeast strains express two different genes capable of coding for functional, yet distinct, VDAC channels. If VDAC were an oligomeric...
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