Article
Phenotypic screening of mutations in Pmr1, the yeast secretory pathway Ca2+/Mn2+-ATPase, reveals residues critical for ion selectivity and transport.
The Journal of biological chemistry - 4 Aug 2000
Wei Y, Chen J, Rosas G, Tompkins D A, Holt P A, Rao R
Abstract excerpt
Thirty-five mutations were generated in the yeast secretory pathway/Golgi ion pump, Pmr1, targeting oxygen-containing side chains within the predicted transmembrane segments M4, M5, M6, M7, and M8, likely to be involved in coordination of Ca(2+) and Mn(2+) ions. Mutants were expressed in low copy number in a yeast strain devoid of endogenous Ca(2+) pumps and screened for loss of Ca(2+) and Mn(2+) transport on the...
Topics
- ATP-Binding Cassette Transporters
- Adenosine Triphosphatases
- Amino Acid Sequence
- Binding Sites
- Biological Transport
- Calcium
- Calcium-Transporting ATPases
- Egtazic Acid
- Fungal Proteins
- Golgi Apparatus