Article
Cloning and characterization of the gene encoding PspPI methyltransferase from the Antarctic psychrotroph Psychrobacter sp. strain TA137. Predicted interactions with DNA and organization of the variable region.
Gene - 15 Sept 1997
Rina M, Caufrier F, Markaki M, Mavromatis K, Kokkinidis M, Bouriotis V
Abstract excerpt
The gene (pspPIM) encoding the PspPI DNA methyltransferase (MTase) associated with the PspPI restriction-modification (R-M) system (5'-GGNCC-3') of Psychrobacter species TA137 has been cloned and expressed in E. coli, and its nucleotide (nt) sequence has been determined. The coding region was 124...
Topics
- Amino Acid Sequence
- Antarctic Regions
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- Conserved Sequence
- DNA-Cytosine Methylases
- Genes, Bacterial
- Genetic Variation
- Gram-Negative Aerobic Bacteria
- Molecular Sequence Data
- Open Reading Frames
- Restriction Mapping