Article
Structure and function of an antifreeze polypeptide from ocean pout, Macrozoarces americanus: role of glutamic acid residues in protein stability and antifreeze activity by site-directed mutagenesis.
Protein engineering - 1 Dec 1991
Li X M, Hew C L
Abstract excerpt
The successful expression and purification of the recombinant ocean pout antifreeze polypeptide (rAFP) in Escherichia coli have enabled the study of its structure-function relationship by site-directed mutagenesis. The role of carboxyl groups at Glu23 and Glu36 of the rAFP was probed by replacing these residues with either glutamine or alanine residues as both single and double mutants. The AFP mutants were...
Topics
- Alanine
- Amino Acid Sequence
- Amino Acids
- Animals
- Antifreeze Proteins
- Base Sequence
- Circular Dichroism
- Escherichia coli
- Fishes
- Freezing
- Gene Expression Regulation, Bacterial