Article
Production of type 1 fimbriae by Escherichia coli HB101.
Microbial pathogenesis - 1 Jun 1991
Elliott S J, Nandapalan N, Chang B J
Abstract excerpt
Escherichia coli HB101 is frequently used as a host in the cloning of bacterial virulence genes because of its reported lack of virulence determinants such as fimbriae, adhesins and haemagglutinins. However, passage of HB101 in standing broth culture rapidly induced the production of fimbriae which mediated adhesion to HEp-2 cells and mannose-sensitive haemagglutination of human and guinea-pig erythrocytes....
Topics
- Animals
- Bacterial Adhesion
- Escherichia coli
- Fimbriae, Bacterial
- Genetic Variation
- Guinea Pigs
- Hemagglutination
- Mannose
- Microscopy, Electron
- Negative Staining
- Serial Passage