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Article

Type 1 fimbriae mutants of Escherichia coli K12: characterization of recognized afimbriate strains and construction of new fim deletion mutants.

Molecular microbiology - 1 Jun 1991

Blomfield I C, McClain M S, Eisenstein B I

Abstract excerpt

We have used Southern hybridization analysis to characterize the extent of fim homology in recognized type 1 fimbriae mutants of Escherichia coli K12, including strains HB101, P678-54, and VL584. We have found extensive homology in strain HB101, and confirm that P678-54 lacks the majority of fim DNA. Strain VL584 contains a deletion of the entire fim region. We have used a new allelic exchange procedure to...

Topics

Blotting, Southern
DNA Mutational Analysis
Escherichia coli
Fimbriae, Bacterial
Mutation
Plasmids
Recombination, Genetic
Restriction Mapping

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 1686292
DOI: 10.1111/j.1365-2958.1991.tb00790.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1686292-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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