Article
Genetic characterization of Escherichia coli type 1 pilus adhesin mutants and identification of a novel binding phenotype.
Journal of bacteriology - 1 Jul 2000
Hamrick T S, Harris S L, Spears P A, Havell E A, Horton J R, Russell P W, Orndorff P E
Abstract excerpt
Five Escherichia coli type 1 pilus mutants that had point mutations in fimH, the gene encoding the type 1 pilus adhesin FimH, were characterized. FimH is a minor component of type 1 pili that is required for the pili to bind and agglutinate guinea pig erythrocytes in a mannose-inhibitable manner. Point mutations were located by DNA sequencing and deletion mapping. All mutations mapped within the signal sequence...
Topics
- Adhesins, Bacterial
- Adhesins, Escherichia coli
- Animals
- Bacterial Adhesion
- Base Sequence
- Chromosome Mapping
- Erythrocytes
- Escherichia coli
- Fimbriae Proteins
- Guinea Pigs
- Hemagglutination Tests
- Macrophages
- Mice