Article
Activity of chloramphenicol acetyltransferase overproduced in E. coli with wild-type and mutant GroEL.
Biochemistry international - 1 Sept 1991
Kim H B, Kang C
Abstract excerpt
The homo-oligomeric protein chloramphenicol acetyltransferase (CAT) has previously been shown to interact with a chaperone GroEL in vitro, suggesting a possible involvement of GroEL in CAT assembly. CAT was overproduced to various levels in the presence and absence of GroEL overproduction, and in groEL mutants. CAT was accumulated to 9-45% of total cellular protein in a fully soluble form, without formation of...
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