Article
Activity of chloramphenicol acetyltransferase overproduced in E. coli with wild-type and mutant GroEL.
Biochemistry international - 1 Sept 1991
Kim H B, Kang C
Abstract excerpt
The homo-oligomeric protein chloramphenicol acetyltransferase (CAT) has previously been shown to interact with a chaperone GroEL in vitro, suggesting a possible involvement of GroEL in CAT assembly. CAT was overproduced to various levels in the presence and absence of GroEL overproduction, and in...
Topics
- Bacterial Proteins
- Chaperonin 60
- Chloramphenicol O-Acetyltransferase
- Culture Media
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Heat-Shock Proteins
- Hot Temperature
- Mutation
- Plasmids