Article
Crystallization of type I chloramphenicol acetyltransferase: an approach based on the concept of ionic strength reducers.
Acta crystallographica. Section D, Biological crystallography - 1 Jan 2000
Andreeva A E, Borissova B E, Mironova R, Glykos N M, Kotsifaki D, Ivanov I, Krysteva M, Kokkinidis M
Abstract excerpt
Chloramphenicol acetyltransferase (CAT) is responsible for bacterial resistance to chloramphenicol. It catalyzes inactivation of the antibiotic by acetyl-group transfer from acetyl CoA to one or both hydroxyl groups of chloramphenicol. Type I CAT possesses some unique properties which are not observed in other CAT variants. Type I CAT overexpressed in Escherichia coli was purified and crystals with a resolution...
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