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Article

Expression of a streptomycete leaderless mRNA encoding chloramphenicol acetyltransferase in Escherichia coli.

Journal of bacteriology - 1 Nov 1997

Wu C J, Janssen G R

Abstract excerpt

The chloramphenicol acetyltransferase (cat) gene from Streptomyces acrimycini encodes a leaderless mRNA. Expression of the cat coding sequence as a leaderless mRNA from a modified lac promoter resulted in chloramphenicol resistance in Escherichia coli. Transcript mapping with nuclease S1 confirme...

Topics

Chloramphenicol
Chloramphenicol O-Acetyltransferase
Chloramphenicol Resistance
Codon, Initiator
Escherichia coli
Lac Operon
Mutation
Promoter Regions, Genetic
Protein Biosynthesis
RNA
RNA, Bacterial
RNA, Messenger

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Recombinant Fusion Proteins

Streptomyces

Source

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Identifiers

PMID: 9352935
DOI: 10.1128/jb.179.21.6824-6830.1997
PMCID: PMC179614

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9352935-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Expression of a streptomycete leaderless mRNA encoding chloramphenicol acetyltransferase in Escherichia coli.PMID 9352935Read ↗

Constitutive expression of cat-86 associated with a change in the transcription start point.Semantic proximity Read ↗

Initiation of protein synthesis from a termination codon.Semantic proximity Read ↗

Chloramphenicol-induced stabilization of cat messenger RNA in Bacillus subtilis.Semantic proximity Read ↗

Effect of 3' terminal codon pairs with different frequency of occurrence on the expression of cat gene in Escherichia coli.Semantic proximity Read ↗

Insertional re-activation of a chloramphenicol acetyltransferase misfolding mutant protein.Semantic proximity Read ↗

Shine-Dalgarno sequence enhances the efficiency of lacZ repression by artificial anti-lac antisense RNAs in Escherichia coli.Semantic proximity Read ↗

Translation initiation with GUC codon in the archaeon Halobacterium salinarum: implications for translation of leaderless mRNA and strict correlation between translation initiation and presence of mRNA.Semantic proximity Read ↗

Study of heterogeneity of chloramphenicol acetyltransferase (CAT) genes in streptococci and enterococci by polymerase chain reaction: characterization of a new CAT determinant.Semantic proximity Read ↗

Activity of chloramphenicol acetyltransferase overproduced in E. coli with wild-type and mutant GroEL.Semantic proximity Read ↗

Lack of a 5' non-coding region in Tn1721 encoded tetR mRNA is associated with a low efficiency of translation and a short half-life in Escherichia coli.Semantic proximity Read ↗

Lysine-induced premature transcription termination in the lysC operon of Bacillus subtilis.Semantic proximity Read ↗

The bldA-encoded tRNA is poorly expressed in the bldI mutant of Streptomyces coelicolor A3(2).Semantic proximity Read ↗

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