Article
Construction and use of retroviral vectors encoding the toxic gene barnase.
Molecular therapy : the journal of the American Society of Gene Therapy - 1 Oct 2006
Agarwal Sumit, Nikolai Bryan, Yamaguchi Tomoyuki, Lech Patrycja, Somia Nikunj V
Abstract excerpt
Suicide genes for negative selection of cells have been powerful tools in somatic cell genetic studies and in gene therapy. Here we report on the construction, characterization, and utilization of retroviral vectors encoding barnase, a ribonuclease from Bacillus amyloliquefaciens, expression of which results in apoptosis of transduced mammalian cells. High-titer viral vector production was enabled by expression...
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