Article
Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.
Nucleic acids research - 25 Jun 1990
Morgenstern J P, Land H
Abstract excerpt
We report the development of an advanced system for transfer and expression of exogenous genes in mammalian cells based on Moloney murine leukemia virus (Mo MuLV). Extensive deletion/mutagenesis analysis to identify cis-acting signals involved in virus transmission has led to the design of a fami...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- DNA Mutational Analysis
- Drug Resistance, Microbial
- Exons
- Gene Expression Regulation
- Genes
- Genes, gag
- Genetic Markers
- Genetic Vectors
- Molecular Sequence Data
- Moloney murine leukemia virus
- Mutation
- Repetitive Sequences, Nucleic Acid
- Sequence Homology, Nucleic Acid
- Transfection