Article
A novel vector for direct cloning PCR fragments by positive selection based on the lethal barnase.
Molecular biology reports - 1 Sept 2009
You Leiming, Weng Haibo, Chen Zhankuan, Wang Aiping, Xu Weihua, Wang Minge, Dong Ziming
Abstract excerpt
A novel vector for direct PCR fragments cloning by positive selection, pBN, was constructed based on the lethal barnase from Bacillus amyloliquefaciens. Barnase was modified by inserting an additional insert at a pivotal Ile-54 site, which could take crucial affect on protein structure and absolute activity. The lacZ' expressing cassette of pUC19 was replaced by the modified barnase under the NptIII promoter....
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