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Article

A novel vector for direct cloning PCR fragments by positive selection based on the lethal barnase.

Molecular biology reports - 1 Sept 2009

You Leiming, Weng Haibo, Chen Zhankuan, Wang Aiping, Xu Weihua, Wang Minge, Dong Ziming

Abstract excerpt

A novel vector for direct PCR fragments cloning by positive selection, pBN, was constructed based on the lethal barnase from Bacillus amyloliquefaciens. Barnase was modified by inserting an additional insert at a pivotal Ile-54 site, which could take crucial affect on protein structure and absolu...

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Share to Bacterial Proteins
Share to Cloning, Molecular
Share to Genetic Vectors

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutagenesis, Site-Directed

Share to Polymerase Chain Reaction

Share to Protein Structure, Secondary

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Share to Selection, Genetic

Source

Identifiers

PMID: 18979229
DOI: 10.1007/s11033-008-9382-5

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-18979229-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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