Article
Ammonia channel couples glutaminase with transamidase reactions in GatCAB.
Science (New York, N.Y.) - 30 Jun 2006
Nakamura Akiyoshi, Yao Min, Chimnaronk Sarin, Sakai Naoki, Tanaka Isao
Abstract excerpt
The formation of glutaminyl transfer RNA (Gln-tRNA(Gln)) differs among the three domains of life. Most bacteria employ an indirect pathway to produce Gln-tRNA(Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB) that acts on the misacylated Glu-tRNA(Gln). Here, we describe a series of crystal structures of intact GatCAB from Staphylococcus aureus in the apo form and in the complexes with glutamine,...
Topics
- Adenosine Diphosphate
- Amino Acid Sequence
- Aminoacyltransferases
- Ammonia
- Apoenzymes
- Asparagine
- Base Pairing
- Catalytic Domain
- Crystallography, X-Ray
- Glutaminase
- Glutamine