Article
Insertion mutagenesis of the yeast Candida famata (Debaryomyces hansenii) by random integration of linear DNA fragments.
Current genetics - 1 Sept 2006
Dmytruk Kostyantyn V, Voronovsky Andriy Y, Sibirny Andriy A
Abstract excerpt
The feasibility of using random insertional mutagenesis to isolate mutants of the flavinogenic yeast Candida famata was explored. Mutagenesis was performed by transformation of the yeast with an integrative plasmid containing the Saccharomyces cerevisiae LEU2 gene as a selective marker. The addition of restriction enzyme together with the plasmid (restriction enzyme-mediated integration, REMI) increased the...
Topics
- Base Sequence
- Candida
- DNA, Fungal
- Genes, Fungal
- Iron
- Mutagenesis, Insertional
- Phenotype
- Plasmids
- Riboflavin
- Saccharomycetales
- Transformation, Genetic