Article
Identification of the genes affecting the regulation of riboflavin synthesis in the flavinogenic yeast Pichia guilliermondii using insertion mutagenesis.
FEMS yeast research - 1 May 2011
Boretsky Yuriy R, Pynyaha Yuriy V, Boretsky Volodymyr Y, Fedorovych Dariya V, Fayura Lyubov R, Protchenko Olha, Philpott Caroline C, Sibirny Andriy A
Abstract excerpt
Pichia guilliermondii is a representative of a group of so-called flavinogenic yeast species that overproduce riboflavin (vitamin B(2)) in response to iron limitation. Using insertion mutagenesis, we isolated P. guilliermondii mutants overproducing riboflavin. Analysis of nucleotide sequence of recombination sites revealed that insertion cassettes integrated into the genome disrupting P. guilliermondii genes...
Topics
- DNA, Fungal
- GTP Cyclohydrolase
- Genes, Fungal
- Hydrogen Peroxide
- Iron
- Mutagenesis, Insertional
- Phenotype
- Pichia
- Riboflavin
- Sequence Deletion
- Temperature