Article
Cloning and Transplantation of the Mesoplasma florum Genome.
ACS synthetic biology - 19 Jan 2018
Baby Vincent, Labroussaa Fabien, Brodeur Joëlle, Matteau Dominick, Gourgues Géraldine, Lartigue Carole, Rodrigue Sébastien
Abstract excerpt
Cloning and transplantation of bacterial genomes is a powerful method for the creation of engineered microorganisms. However, much remains to be understood about the molecular mechanisms and limitations of this approach. We report the whole-genome cloning of Mesoplasma florum in Saccharomyces cerevisiae, and use this model to investigate the impact of a bacterial chromosome in yeast cells. Our results indicate...
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