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Article

Cloning and Transplantation of the Mesoplasma florum Genome.

ACS synthetic biology - 19 Jan 2018

Baby Vincent, Labroussaa Fabien, Brodeur Joëlle, Matteau Dominick, Gourgues Géraldine, Lartigue Carole, Rodrigue Sébastien

Abstract excerpt

Cloning and transplantation of bacterial genomes is a powerful method for the creation of engineered microorganisms. However, much remains to be understood about the molecular mechanisms and limitations of this approach. We report the whole-genome cloning of Mesoplasma florum in Saccharomyces cerevisiae, and use this model to investigate the impact of a bacterial chromosome in yeast cells. Our results indicate...

Topics

Bacterial Proteins
Cloning, Molecular
Deoxyribonucleases, Type II Site-Specific
Entomoplasmataceae
Gene Expression Profiling
Gene Transfer Techniques
Genome, Bacterial
High-Throughput Nucleotide Sequencing
Hydro-Lyases
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasmids

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Sequence Analysis, DNA

Source

Identifiers

PMID: 28893065
DOI: 10.1021/acssynbio.7b00279

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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