Interactions of the acid and base catalysts on staphylococcal nuclease as studied in a double mutant.

doi:10.1021/bi00239a004

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Article

Interactions of the acid and base catalysts on staphylococcal nuclease as studied in a double mutant.

Biochemistry - 25 Jun 1991

Weber D J, Meeker A K, Mildvan A S

Abstract excerpt

The mechanism of the phosphodiesterase reaction catalyzed by staphylococcal nuclease is believed to involve concerted general acid-base catalysis by Arg-87 and Glu-43. The mutual interactions of Arg-87 and Glu-43 were investigated by comparing kinetic and thermodynamic properties of the single mu...

Topics

Arginine
Binding Sites
Calcium
Catalysis
Circular Dichroism
Enzyme Stability
Glutamates
Glutamic Acid
Kinetics
Manganese
Micrococcal Nuclease
Mutation
Protein Conformation
Staphylococcus
Substrate Specificity
Thermodynamics

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