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Article

Interactions of the acid and base catalysts on staphylococcal nuclease as studied in a double mutant.

Biochemistry - 25 Jun 1991

Weber D J, Meeker A K, Mildvan A S

Abstract excerpt

The mechanism of the phosphodiesterase reaction catalyzed by staphylococcal nuclease is believed to involve concerted general acid-base catalysis by Arg-87 and Glu-43. The mutual interactions of Arg-87 and Glu-43 were investigated by comparing kinetic and thermodynamic properties of the single mutant enzymes E43S (Glu-43 to Ser) and R87G (Arg-87 to Gly) with those of the double mutant, E43S + R87G, in which both...

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Identifiers

PMID: 1676297
DOI: 10.1021/bi00239a004

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Interactions of the acid and base catalysts on staphylococcal nuclease as studied in a double mutant.PMID 1676297Read ↗

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