Article
Energetics of denaturation and m values of staphylococcal nuclease mutants.
Biochemistry - 14 Feb 1995
Carra J H, Privalov P L
Abstract excerpt
In a continuation of an earlier study [Carra, J., Anderson, E., & Privalov, P. (1994) Biochemistry 33, 10842-10850], we used differential scanning calorimetry to measure the enthalpy and heat capacity changes of denaturation for 11 mutant forms of staphylococcal nuclease, including the triple mutant [V66L+G88V+G79S]. Several mutant proteins with m- characteristics of guanidinium chloride denaturation were found...
Topics
- Calorimetry, Differential Scanning
- Circular Dichroism
- Escherichia coli
- Guanidine
- Guanidines
- Hydrogen-Ion Concentration
- Micrococcal Nuclease
- Models, Molecular
- Mutation
- Protein Denaturation
- Protein Folding