Article
Generation of an antibody with enhanced affinity and specificity for its antigen by protein engineering.
Nature - 1 Jan 2000
Roberts S, Cheetham J C, Rees A R
Abstract excerpt
A detailed description of the interactions between an antibody and its epitope is necessary to allow an understanding of the way in which antibodies bind to antigenic surfaces presented by foreign molecules. Ideally this should be done by analysis of crystal structures of antibody-antigen complexes, but so far only two of these are available. An alternative strategy combines molecular modelling with site-directed...
Topics
- Animals
- Antibody Specificity
- Antigen-Antibody Complex
- Base Sequence
- Chickens
- Cloning, Molecular
- Computer Simulation
- Kinetics
- Models, Molecular
- Muramidase
- Mutation
- Protein Conformation