Mapping unprocessed epitopes using deletion mutagenesis of gene fusions.

Abstract excerpt

To locate the antigenic determinant recognized by a monoclonal antibody directed against a baculovirus capsid protein, a series of overlapping deletions of a fusion protein were immunologically screened with the monoclonal antibody. The immunoreactive fusion protein was derived from a restriction...

Topics

• Amino Acid Sequence
• Base Sequence
• Blotting, Western
• Capsid
• Chromosome Deletion
• Chromosome Mapping
• Cloning, Molecular
• Epitopes
• Genetic Vectors
• Methods
• Molecular Sequence Data
• Mutation
• Recombinant Fusion Proteins