Article
c-met is amplified but not mutated in a cell line with an activated met tyrosine kinase.
Oncogene - 1 Apr 1991
Ponzetto C, Giordano S, Peverali F, Della Valle G, Abate M L, Vaula G, Comoglio P M
Abstract excerpt
The putative tyrosine kinase receptor encoded by the oncogene c-met is activated (tyrosine-phosphorylated in vivo) in the human gastric carcinoma cell line GTL-16. The corresponding gene is amplified and over-expressed. In this study we show that c-met is part of an amplification unit measuring more than 3000 kb. The multiple copies of the amplicon are located on a novel chromosome different from chromosome 7. We...
Topics
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- Cell Line
- Gene Amplification
- Genetic Markers
- Humans
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length