Article
Site-directed mutagenesis of the beta subunit of tryptophan synthase from Salmonella typhimurium. Role of active site glutamic acid 350.
The Journal of biological chemistry - 25 Apr 1991
Kayastha A M, Sawa Y, Nagata S, Miles E W
Abstract excerpt
To investigate the functional role of glutamic acid 350 in the active site of the beta subunit of tryptophan synthase from Salmonella typhimurium, we have replaced this residue by glutamine or alanine by use of site-directed mutagenesis. The mutant alpha 2 beta 2 complexes were expressed, purifie...
Topics
- Amino Acids
- Binding Sites
- Circular Dichroism
- Fluorescence Polarization
- Glutamates
- Glutamic Acid
- Mutagenesis, Site-Directed
- Mutation
- Salmonella typhimurium
- Spectrum Analysis
- Tryptophan Synthase