Article
Threonine 183 and adjacent flexible loop residues in the tryptophan synthase alpha subunit have critical roles in modulating the enzymatic activities of the beta subunit in the alpha 2 beta 2 complex.
The Journal of biological chemistry - 15 Apr 1992
Yang X J, Miles E W
Abstract excerpt
This study investigates the catalytic and allosteric roles of a flexible loop in the tryptophan synthase alpha 2 beta 2 complex. This loop connects helix 6 and strand 6 in the alpha subunit, an 8-fold alpha/beta barrel polypeptide. We have engineered three mutations in this disordered loop: a del...
Topics
- Amino Acid Sequence
- Catalysis
- Electrophoresis, Polyacrylamide Gel
- Fluorescence
- Glycerophosphates
- Hydrolysis
- Indoles
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation
- Salmonella typhimurium
- Serine
- Threonine
- Tryptophan Synthase