Monovalent cations partially repair a conformational defect in a mutant tryptophan synthase alpha 2 beta 2 complex (beta-E109A).

doi:10.1074/jbc.270.29.17333

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Article

Monovalent cations partially repair a conformational defect in a mutant tryptophan synthase alpha 2 beta 2 complex (beta-E109A).

The Journal of biological chemistry - 21 Jul 1995

Ruvinov S B, Ahmed S A, McPhie P, Miles E W

Abstract excerpt

We are using the tryptophan synthase alpha 2 beta 2 complex as a model system to investigate how ligands, protein-protein interaction, and mutations regulate enzyme activity, reaction specificity, and substrate specificity. The rate of conversion of L-serine and indole to L-tryptophan by the beta...

Topics

Binding Sites
Cesium
Crystallography
Lithium
Mutation
Protein Conformation
Pyridoxal Phosphate
Quaternary Ammonium Compounds
Spectrophotometry
Structure-Activity Relationship
Tryptophan Synthase

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