Article
Effect of temperature on the expression of wild-type and thermostable mutants of kanamycin nucleotidyltransferase in Escherichia coli.
Protein expression and purification - 1 Feb 1991
Liao H H
Abstract excerpt
The expression of kanamycin nucleotidyltransferase (KNTase) in Escherichia coli results in different forms of the protein, depending on the temperature; soluble active enzyme is synthesized at 23 degrees C but the protein is mostly aggregated and inactive in inclusion bodies when made at 37 degrees C. However, active enzyme can be recovered by solubilization of the inclusion bodies with 8 M urea followed by...
Topics
- Base Sequence
- DNA, Bacterial
- Enzyme Stability
- Escherichia coli
- Gene Expression
- Inclusion Bodies
- Molecular Sequence Data
- Mutation
- Nucleotidyltransferases
- Plasmids
- Protein Conformation