Thermal denaturation of beta-galactosidase and of two site-specific mutants.

doi:10.1021/bi00501a019

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Article

Thermal denaturation of beta-galactosidase and of two site-specific mutants.

Biochemistry - 11 Dec 1990

Edwards R A, Jacobson A L, Huber R E

Abstract excerpt

The thermal denaturation of wild-type beta-galactosidase and two beta-galactosidases with substitutions at the active site was studied by kinetics, differential scanning calorimetry, electrophoresis, molecular exclusion chromatography, and circular dichroism. From the results, a model is develope...

Topics

Binding Sites
Calorimetry, Differential Scanning
Circular Dichroism
Hot Temperature
Ligands
Magnesium
Mutation
Protein Conformation
Protein Denaturation
Structure-Activity Relationship
Thermodynamics
Thiogalactosides
beta-Galactosidase

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