Article
Mg2+ and a key lysine modulate exchange activity of eukaryotic translation elongation factor 1B alpha.
The Journal of biological chemistry - 14 Jul 2006
Pittman Yvette R, Valente Louis, Jeppesen Mads Gravers, Andersen Gregers Rom, Patel Smita, Kinzy Terri Goss
Abstract excerpt
To sustain efficient translation, eukaryotic elongation factor B alpha (eEF1B alpha) functions as the guanine nucleotide exchange factor for eEF1A. Stopped-flow kinetics using 2'-(or 3')-O-N-methylanthraniloyl (mant)-GDP showed spontaneous release of nucleotide from eEF1A is extremely slow and accelerated 700-fold by eEF1B alpha. The eEF1B alpha-stimulated reaction was inhibited by Mg2+ with a K(1/2) of 3.8 mM....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.