Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology.

Nature methods - 1 Mar 2007

Mitani Yasumasa, Lezhava Alexander, Kawai Yuki, Kikuchi Takeshi, Oguchi-Katayama Atsuko, Kogo Yasushi, Itoh Masayoshi, Miyagi Toru, Takakura Hideki, Hoshi Kanako, Kato Chiaki, Arakawa Takahiro, Shibata Kazuhiro, Fukui Kenji, Masui Ryoji, Kuramitsu Seiki, Kiyotani Kazuma, Chalk Alistair, Tsunekawa Katsuhiko, Murakami Masami, Kamataki Tetsuya, Oka Takanori, Shimada Hiroshi, Cizdziel Paul E, Hayashizaki Yoshihide

Abstract excerpt

We developed a rapid single nucleotide polymorphism (SNP) detection system named smart amplification process version 2 (SMAP 2). Because DNA amplification only occurred with a perfect primer match, amplification alone was sufficient to identify the target allele. To achieve the requisite fidelity...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Algorithms
Share to Base Pair Mismatch

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Mutational Analysis

Share to DNA Primers

Share to Nucleic Acid Amplification Techniques

Share to Software

Share to Suppression, Genetic

Share to Temperature

Source

Identifiers

PMID: 17322893
DOI: 10.1038/nmeth1007

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-17322893-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology.PMID 17322893Read ↗

Detection of SNP by the isothermal smart amplification method.Semantic proximity Read ↗

Concentration-Bias-Free Discrimination of Single Nucleotide Variants Using Isothermal Nucleic Acid Amplification and Mismatch-Guided DNA Assembly.Semantic proximity Read ↗

DNA nucleic acid sequence-based amplification-based genotyping for polymorphism analysis.Semantic proximity Read ↗

Harnessing Non-standard Nucleic Acids for Highly Sensitive Icosaplex (20-Plex) Detection of Microbial Threats for Environmental SurveillanceSemantic proximity Read ↗

Recombinase polymerase amplification for single nucleotide polymorphism-specific detection of βC variant in sickle cell disease.Semantic proximity Read ↗

Rapid and cost-effective SNP detection method: application of SmartAmp2 to pharmacogenomics research.Semantic proximity Read ↗

A multiplexed, allele-specific recombinase polymerase amplification assay with lateral flow readout for sickle cell disease detection.Semantic proximity Read ↗

Visual DNA detection and SNP genotyping using asymmetric PCR and split DNA enzymes.Semantic proximity Read ↗

Melt analysis of mismatch amplification mutation assays (Melt-MAMA): a functional study of a cost-effective SNP genotyping assay in bacterial models.Semantic proximity Read ↗

Isothermal single nucleotide polymorphism genotyping and direct PCR from whole blood using a novel whole-blood lysis buffer.Semantic proximity Read ↗

Rapid detection of diagnostic targets using isothermal amplification and HyBeacon probes--a homogenous system for sequence-specific detection.Semantic proximity Read ↗

Clinical SNP detection by the SmartAmp method.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.