Article
Optimized MOL-PCR for Characterization of Microbial Pathogens.
Current protocols in cytometry - 6 Jan 2016
Wuyts Véronique, Roosens Nancy H C, Bertrand Sophie, Marchal Kathleen, De Keersmaecker Sigrid C J
Abstract excerpt
Characterization of microbial pathogens is necessary for surveillance, outbreak detection, and tracing of outbreak sources. This unit describes a multiplex oligonucleotide ligation-PCR (MOL-PCR) optimized for characterization of microbial pathogens. With MOL-PCR, different types of markers, like unique sequences, single-nucleotide polymorphisms (SNPs) and indels, can be simultaneously analyzed in one assay. This...
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