Article
Novel multiplex single nucleotide polymorphism-based method for identifying epidemic clones of Listeria monocytogenes.
Applied and environmental microbiology - 1 Sept 2011
Lomonaco Sara, Knabel Stephen J, Dalmasso Alessandra, Civera Tiziana, Bottero Maria Teresa
Abstract excerpt
A novel primer extension-based, multiplex minisequencing assay targeting six highly informative single nucleotide polymorphisms (SNPs) in four virulence genes correctly identified and differentiated all four epidemic clones (ECs) of Listeria monocytogenes and 9 other strains initially misclassified as non-ECs. This assay allows rapid, accurate, and high-throughput screening for all known ECs of L. monocytogenes.
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