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Mining Listeria monocytogenes single nucleotide polymorphism sites to identify the major serotypes using allele-specific multiplex PCR.

International journal of food microbiology - 16 Dec 2020

Zhang Xu, Ling Li, Li Zhiyong, Wang Jufang

Abstract excerpt

Worldwide, Listeria monocytogenes is a common foodborne pathogen and serotyping is necessary for traceability and surveillance. Due to high accuracy and speed, PCR is commonly used for serotyping by targeting specific genes, such as lmo1118 and ORF2110 for 1/2c-3c and 4b-4d-4e, respectively. Single nucleotide polymorphisms (SNPs) are single base alterations at specific loci which are associated with various...

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PMID: 32947145
DOI: 10.1016/j.ijfoodmicro.2020.108885

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