Article
DNA replication in Escherichia coli gyrB(Ts) mutants analysed by flow cytometry.
Research in microbiology - 1 Jan 2000
von Freiesleben U, Rasmussen K V
Abstract excerpt
We have investigated the initiation of DNA replication in Escherichia coli gyrB (Ts) mutants and find that initiations in single cells, even those that occur at non-permissive temperature, are synchronous. Furthermore, our results indicate that the gradual arrest of DNA replication at non-permissive temperature reflects a general decrease in transcription activity and not an initiation-specific function of the...
Topics
- Cell Cycle
- DNA Replication
- DNA Topoisomerases, Type II
- Escherichia coli
- Flow Cytometry
- Mutation
- Temperature
