Temperature-sensitive variants of Saccharomyces cerevisiae iso-1-cytochrome c produced by random mutagenesis of codons 43 to 54.

doi:10.1016/0022-2836(91)80207-b

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Article

Temperature-sensitive variants of Saccharomyces cerevisiae iso-1-cytochrome c produced by random mutagenesis of codons 43 to 54.

Journal of molecular biology - 5 Sept 1991

Wang X H, Pielak G J

Abstract excerpt

In vitro random mutagenesis within the CYC1 gene from the yeast Saccharomyces cerevisiae was used to produce a library of mutants encompassing codons 43 to 54 of iso-1-cytochrome c. This region consists of an evolutionarily conserved structure within an evolutionarily diverse sequence. The librar...

Topics

Base Sequence
Codon
Cytochrome c Group
Cytochromes c
DNA
Deoxyribonucleotides
Gene Library
Molecular Sequence Data
Mutagenesis
Phenotype
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Temperature
Transformation, Genetic

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