The function of the Saccharomyces cerevisiae iso-1-cytochrome c gene is independent of the codon at invariant residue Phe82 when the gene is present on a low-copy-number vector.

doi:10.1093/protein/4.5.575

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Article

The function of the Saccharomyces cerevisiae iso-1-cytochrome c gene is independent of the codon at invariant residue Phe82 when the gene is present on a low-copy-number vector.

Protein engineering - 1 Jun 1991

Hilgen S E, Pielak G J

Abstract excerpt

Phe82 is the most studied invariant residue of cytochrome c. However, the physiological relevance of amino acid substitutions at this position is unclear because previous studies were either performed in vitro (i.e. using purified protein) or in yeast where the gene for the protein is present on a multi-copy vector. Multi-copy vectors yield a level of cytochrome c in yeast that is greater than the wild-type...

Topics

Alleles
Base Sequence
Codon
Coliphages
Cytochrome c Group
Cytochromes c
Gene Amplification
Genes, Fungal
Genetic Variation
Genetic Vectors
Glycerol

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Lactates

Lactic Acid

Molecular Sequence Data

Mutagenesis, Site-Directed

Phenylalanine

Plasmids

Saccharomyces cerevisiae

Source

Open in PubMed

Identifiers

PMID: 1653957
DOI: 10.1093/protein/4.5.575

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1653957-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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