Article
Extragenic suppressors of a translation initiation defect in the cyc1 gene of Saccharomyces cerevisiae.
Biochimie - 1 Dec 1991
Hampsey M, Na J G, Pinto I, Ware D E, Berroteran R W
Abstract excerpt
The cycl-362 allele contains a point mutation that generates an aberrant AUG codon upstream of the normal CYC1 translation initiation codon. Mutants containing this allele express only about 2% of normal iso-1-cytochrome c, presumably due to translation initiation at the upstream AUG, termination at a UAA sequence six codons downstream, and failure to reinitiate at the normal AUG codon two nucleotides later. Both...
Topics
- Alleles
- Base Sequence
- Cytochrome c Group
- Cytochromes c
- Gene Expression Regulation, Fungal
- Genotype
- Molecular Sequence Data
- Mutation
- Protein Biosynthesis
- RNA, Messenger
- Saccharomyces cerevisiae