Article
Role of the surface charges D72 and K8 in the function and structural stability of the cytochrome c from Nostoc sp. PCC 7119.
The FEBS journal - 1 Jul 2005
Lange Christian, Luque Irene, Hervás Manuel, Ruiz-Sanz Javier, Mateo Pedro L, De la Rosa Miguel A
Abstract excerpt
We investigated the role of electrostatic charges at positions D72 and K8 in the function and structural stability of cytochrome c6 from Nostoc sp. PCC 7119 (cyt c6). A series of mutant forms was generated to span the possible combinations of charge neutralization (by mutation to alanine) and charge inversion (by mutation to lysine and aspartate, respectively) in these positions. All forms of cyt c6 were...
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