Article
Overexpression of RPI1, a novel inhibitor of the yeast Ras-cyclic AMP pathway, down-regulates normal but not mutationally activated ras function.
Molecular and cellular biology - 1 Aug 1991
Kim J H, Powers S
Abstract excerpt
A high-copy-number plasmid genomic library was screened for genes that when overexpressed down-regulate Ras protein activity in Saccharomyces cerevisiae. We report on the structure and characterization of one such gene, RPI1, which potentially encodes a novel 46-kDa negative regulator of the Ras-...
Topics
- Amino Acid Sequence
- Base Sequence
- Cyclic AMP
- Fungal Proteins
- GTP-Binding Proteins
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genomic Library
- Genotype
- Molecular Sequence Data
- Oligonucleotide Probes
- Plasmids
- Repressor Proteins
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Suppression, Genetic
- ras Proteins