Article
Effect of a pmr 1 disruption and different signal sequences on the intracellular processing and secretion of Cyamopsis tetragonoloba alpha-galactosidase by Saccharomyces cerevisiae.
Gene - 30 Mar 1993
Harmsen M M, Langedijk A C, van Tuinen E, Geerse R H, Raué H A, Maat J
Abstract excerpt
We fused the yeast-derived sequences encoding the invertase, acid phosphatase and alpha-factor pre- and prepro-signal peptides (SP) to the Cyamopsis tetragonoloba (guar plant) alpha-galactosidase(alpha Gal)-encoding gene and expressed these gene fusions in yeast. Whereas the amount of fusion prot...
Topics
- Base Sequence
- Blotting, Western
- Calcium-Transporting ATPases
- Cloning, Molecular
- DNA, Fungal
- Fungal Proteins
- Genes, Fungal
- Glycosylation
- Isoelectric Focusing
- Microscopy, Immunoelectron
- Molecular Sequence Data
- Mutation
- Plants
- Proprotein Convertases