Article
Type A and B RNase P RNAs are interchangeable in vivo despite substantial biophysical differences.
EMBO reports - 1 Apr 2006
Wegscheid Barbara, Condon Ciarán, Hartmann Roland K
Abstract excerpt
We show that structural type A and B bacterial ribonuclease P (RNase P) RNAs can fully replace each other in vivo despite the many reported differences in their biogenesis, biochemical/biophysical properties and enzyme function in vitro. Our findings suggest that many of the reported idiosyncrasies of type A and B enzymes either do not reflect the in vivo situation or are not crucial for RNase P function in vivo,...
Topics
- Bacillus subtilis
- Base Sequence
- Cell Proliferation
- Escherichia coli
- Holoenzymes
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA, Bacterial
- Recombinant Proteins
- Ribonuclease P
- Transcription, Genetic