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Article

The particle inflow gun can be used to co-transform Paramecium using Tungsten particles.

The Journal of eukaryotic microbiology - 1 Jan 2000

Fraga Dean, Keenan Erica, Hendel Emily, Nair Anjali, Schofield Whit

Abstract excerpt

A particle inflow gun (PIG) was constructed and tested for its utility to transform Paramecium using tungsten or gold as the DNA carrier particle. In the first set of experiments we transformed Paramecium with a plasmid containing the neomycin-resistance gene, obtaining a transformation efficiency of 0.31+/-0.14% (mean+/-SD) for tungsten particles and 1.30+/-0.29% for gold particles. Plasmid DNA precipitated upon...

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Identifiers

PMID: 16441576
DOI: 10.1111/j.1550-7408.2005.00067.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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