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Article

Stable transfection of Acanthamoeba castellanii.

Biochimica et biophysica acta - 22 Mar 2005

Peng Zhihua, Omaruddin Romaica, Bateman Erik

Abstract excerpt

A simple method for stable transfection of Acanthamoeba castellanii using plasmids which confer resistance to neomycin G418 is described. Expression of neomycin phosphotransferase is driven by the Acanthamoeba TBP gene promoter, and can be monitored by cell growth in the presence of neomycin G418 or by Western blot analysis. Transfected cells can be passaged in the same manner as control cells and can be induced...

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Source

Identifiers

PMID: 15777844
DOI: 10.1016/j.bbamcr.2004.08.014

Genes

TBP

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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